Insights into mitotic checkpoint by integrating CRISPR and RNAi.

Published in: Molecular & cellular oncology (2019)

Our recent study of the mitotic checkpoint protein BUB1 (budding uninhibited by benzimidazoles 1) revealed several apparent BUB1 knock-out cell lines expressing low levels of BUB1 protein sufficient to support spindle assembly checkpoint activity. This rings alarm bells on the application of CRISPR technology.

Keyphrases

cell cycle
dna damage
crispr cas
genome editing
genome wide
cell proliferation
protein protein
amino acid
single cell
dna methylation
magnetic resonance imaging
small molecule
diffusion weighted imaging