Enhancement of Bone Marrow-Derived Mesenchymal Stem Cell Osteogenesis and New Bone Formation in Rats by Obtusilactone A.
Yi-Hsiung LinChung-Yi ChenLiang-Yin ChouChung-Hwan ChenLin KangChau-Zen WangPublished in: International journal of molecular sciences (2017)
The natural pure compound obtusilactone A (OA) was identified in Cinnamomum kotoense Kanehira & Sasaki, and shows effective anti-cancer activity. We studied the effect of OA on osteogenesis of bone marrow-derived mesenchymal stem cells (BMSCs). OA possesses biocompatibility, stimulates Alkaline Phosphatase (ALP) activity and facilitates mineralization of BMSCs. Expression of osteogenesis markers BMP2, Runx2, Collagen I, and Osteocalcin was enhanced in OA-treated BMSCs. An in vivo rat model with local administration of OA via needle implantation to bone marrow-residing BMSCs revealed that OA increased the new bone formation and trabecular bone volume in tibias. Micro-CT images and H&E staining showed more trabecular bone at the needle-implanted site in the OA group than the normal saline group. Thus, OA confers an osteoinductive effect on BMSCs via induction of osteogenic marker gene expression, such as BMP2 and Runx2 expression and subsequently elevates ALP activity and mineralization, followed by enhanced trabecular bone formation in rat tibias. Therefore, OA is a potential osteoinductive drug to stimulate new bone formation by BMSCs.
Keyphrases
- mesenchymal stem cells
- bone marrow
- knee osteoarthritis
- bone mineral density
- gene expression
- bone regeneration
- umbilical cord
- poor prognosis
- transcription factor
- dna methylation
- postmenopausal women
- stem cells
- magnetic resonance
- magnetic resonance imaging
- emergency department
- risk assessment
- ultrasound guided
- convolutional neural network
- binding protein
- positron emission tomography
- tissue engineering
- contrast enhanced
- electronic health record
- flow cytometry