Inner limiting Membrane Peel Extends In vivo Calcium Imaging of Retinal Ganglion Cell Activity Beyond the Fovea in Non-Human Primate.

This research presents a groundbreaking advancement in visual neuroscience through the development of a novel technique involving the peeling of the inner limiting membrane (ILM) in conjunction with intravitreal injection to expand functional recording capabilities in the living primate eye. By utilizing high-resolution retinal imaging coupled with a viral vector-mediated expression of the calcium indicator GCaMP, the study achieves unprecedented visualization and assessment of retinal ganglion cell (RGC) activity at single-cell resolution. Importantly, the technique enables recording from regions of the retina previously inaccessible, significantly broadening the area for calcium imaging beyond the fovea. The results demonstrate stable RGC function post-procedure, suggesting minimal impact on retinal physiology. This innovative approach holds significant promise for diverse applications in visual neuroscience, including pre-clinical evaluation of retinal function, detection of vision loss, and evaluation of therapeutic interventions. Overall, the study represents a major step forward in understanding and potentially treating retinal degenerative disorders, offering new avenues for research and development in vision restoration.