Differentiation of the human PAX7-positive myogenic precursors/satellite cell lineage in vitro.
Ziad Al TanouryJyoti RaoOlivier TassyBénédicte GobertSvetlana GaponJean-Marie GarnierErica WagnerAurore HickArielle HallEmanuela GussoniOlivier PourquiePublished in: Development (Cambridge, England) (2020)
Satellite cells (SC) are muscle stem cells that can regenerate adult muscles upon injury. Most SC originate from PAX7+ myogenic precursors set aside during development. Although myogenesis has been studied in mouse and chicken embryos, little is known about human muscle development. Here, we report the generation of human induced pluripotent stem cell (iPSC) reporter lines in which fluorescent proteins have been introduced into the PAX7 and MYOG loci. We use single cell RNA sequencing to analyze the developmental trajectory of the iPSC-derived PAX7+ myogenic precursors. We show that the PAX7+ cells generated in culture can produce myofibers and self-renew in vitro and in vivo Together, we demonstrate that cells exhibiting characteristics of human fetal satellite cells can be produced in vitro from iPSC, opening interesting avenues for muscular dystrophy cell therapy. This work provides significant insights into the development of the human myogenic lineage.
Keyphrases
- stem cells
- single cell
- endothelial cells
- induced pluripotent stem cells
- induced apoptosis
- cell therapy
- skeletal muscle
- cell cycle arrest
- pluripotent stem cells
- rna seq
- high glucose
- endoplasmic reticulum stress
- gene expression
- cell proliferation
- oxidative stress
- dna methylation
- crispr cas
- high throughput
- duchenne muscular dystrophy
- pi k akt
- stress induced
- label free