Mitochondria Participate in Chemoresistance to Cisplatin in Human Ovarian Cancer Cells.
Luca X ZampieriDebora GrassoCaroline BouzinDavide BrusaRodrigue RossignolPierre SonveauxPublished in: Molecular cancer research : MCR (2020)
Ovarian cancer is an aggressive disease that affects about 300,000 patients worldwide, with a yearly death count of about 185,000. Following surgery, treatment involves adjuvant or neoadjuvant administration of taxane with platinum compounds cisplatin or carboplatin, which alkylate DNA through the same chemical intermediates. However, although platinum-based therapy can cure patients in a number of cases, a majority of them discontinues treatment owing to side effects and to the emergence of resistance. In this study, we focused on resistance to cisplatin and investigated whether metabolic changes could be involved. As models, we used matched pairs of cisplatin-sensitive (SKOV-3 and COV-362) and cisplatin-resistant (SKOV-3-R and COV-362-R) human ovarian carcinoma cells that were selected in vitro following exposure to increasing doses of the chemotherapy. Metabolic comparison revealed that resistant cells undergo a shift toward a more oxidative metabolism. The shift goes along with a reorganization of the mitochondrial network, with a generally increased mitochondrial compartment. More functional mitochondria in cisplatin-resistant compared with cisplatin-sensitive cells were associated to enzymatic changes affecting either the electron transport chain (SKOV-3/SKOV-3-R model) or mitochondrial coupling (COV-362/COV-362-R model). Our findings further indicate that the preservation of functional mitochondria in these cells could be due to an increased mitochondrial turnover rate, suggesting mitophagy inhibition as a potential strategy to tackle cisplatin-resistant human ovarian cancer progression. IMPLICATIONS: Besides classical mechanisms related to drug efflux and target modification, we report that preserving functional mitochondria is a strategy used by human ovarian cancer cells to resist to cisplatin chemotherapy.
Keyphrases
- endothelial cells
- induced apoptosis
- sars cov
- end stage renal disease
- oxidative stress
- cell death
- cell cycle arrest
- induced pluripotent stem cells
- ejection fraction
- newly diagnosed
- respiratory syndrome coronavirus
- pluripotent stem cells
- chronic kidney disease
- prognostic factors
- locally advanced
- endoplasmic reticulum
- peritoneal dialysis
- clinical trial
- stem cells
- squamous cell carcinoma
- coronary artery disease
- endoplasmic reticulum stress
- body composition
- signaling pathway
- hydrogen peroxide
- atrial fibrillation
- lymph node
- bone marrow
- study protocol
- mass spectrometry
- single cell
- replacement therapy
- coronary artery bypass
- circulating tumor cells
- high resolution