Cell-type-specific CRISPRization of mitochondrial DNA using bifunctional biodegradable silica nanoparticles.

Linye JiangBizhong ZhouHuijuan QianHongfeng WangYuxi WangWeijiao FanGuowan ZhengJingyan Ge

Published in: Chemical communications (Cambridge, England) (2023)

We report cell-type-specific and CRISPR/Cas9-mediated mtDNA editing platform by using bifunctional biodegradable silica nanoparticles, which were capable of selective intracellular delivery to CD44-overexpressed cells and subsequent mitochondrial localization, followed by glutathione-responsive biodegradation and release of Cas9/sgRNA to realize precise mtDNA editing.

Keyphrases

crispr cas
mitochondrial dna
genome editing
copy number
drug delivery
induced apoptosis
oxidative stress
cell cycle arrest
highly efficient
cancer therapy
genome wide
high throughput
dna methylation
gene expression
signaling pathway
reactive oxygen species
endoplasmic reticulum stress