The MIR34B/C genomic region contains multiple potential regulators of multiciliogenesis.
Amélie CavardElisa RedmanOlivier MerceySophie AbelanetMagali PlaisantMarie-Jeanne ArguelVirginie MagnoneSandra Ruiz GarcíaGéraldine RiosMarie DeprezKévin LebrigandGilles PonzioIgnacio CaballeroPascal BarbryLaure-Emmanuelle ZaragosiBrice MarcetPublished in: FEBS letters (2023)
The MIR449 genomic locus encompasses several regulators of multiciliated cell (MCC) formation (multiciliogenesis). The miR-449 homologs miR-34b/c represent additional regulators of multiciliogenesis that are transcribed from another locus. Here, we characterized the expression of BTG4, LAYN, and HOATZ, located in the MIR34B/C locus using single-cell RNA-seq and super-resolution microscopy from human, mouse, or pig multiciliogenesis models. BTG4, LAYN, and HOATZ transcripts were expressed in both precursors and mature MCCs. The Layilin/LAYN protein was absent from primary cilia, but it was expressed in apical membrane regions or throughout motile cilia. LAYN silencing altered apical actin cap formation and multiciliogenesis. HOATZ protein was detected in primary cilia or throughout motile cilia. Altogether, our data suggest that the MIR34B/C locus may gather potential actors of multiciliogenesis.
Keyphrases
- single cell
- rna seq
- high throughput
- cell proliferation
- long non coding rna
- genome wide association study
- transcription factor
- poor prognosis
- binding protein
- endothelial cells
- long noncoding rna
- amino acid
- copy number
- high resolution
- big data
- mesenchymal stem cells
- high speed
- artificial intelligence
- single molecule
- cell therapy
- small molecule
- bone marrow
- machine learning
- climate change