m6A mRNA methylation controls autophagy and adipogenesis by targeting Atg5 and Atg7.
Xinxia WangRuifan WuYouhua LiuYuanling ZhaoZhen BiYongxi YaoQing LiuHailing ShiFengqin WangYizhen WangPublished in: Autophagy (2019)
3-MA: 3-methyladenine; ACTB: actin, beta; ATG: autophagy-related; Baf A1: bafilomycin A1; CEBPA: CCAAT/enhancer binding protein (C/EBP), alpha; CEBPB: CCAAT/enhancer binding protein (C/EBP), beta; FABP4: fatty acid binding protein 4, adipocyte; FTO: fat mass and obesity associated; HFD: high-fat diet; LC-MS/MS: liquid chromatography-tandem mass spectrometry; MAP1LC3B/LC3: microtubule-associated protein 1 light chain 3 beta; m6A: N6-methyladenosine; MEFs: mouse embryo fibroblasts; MeRIP-qPCR: methylated RNA immunoprecipitation-qPCR; PPARG: peroxisome proliferator activated receptor gamma; RIP: RNA-immunoprecipitation; SAT: subcutaneous adipose tissue; siRNA: small interfering RNA; SQSTM1/p62: sequestosome 1; TEM: transmission electron microscopy; ULK1: unc-51 like kinase 1; VAT: visceral adipose tissue; WAT: white adipose tissue; YTHDF: YTH N6-methyladenosine RNA binding protein.
Keyphrases
- binding protein
- adipose tissue
- high fat diet
- insulin resistance
- liquid chromatography tandem mass spectrometry
- simultaneous determination
- high fat diet induced
- fatty acid
- electron microscopy
- cell death
- type diabetes
- solid phase extraction
- metabolic syndrome
- nucleic acid
- skeletal muscle
- oxidative stress
- endoplasmic reticulum stress
- signaling pathway
- weight loss
- body mass index
- dna methylation
- liquid chromatography
- high density
- transcription factor
- cell migration