Sequential changes in histone modifications shape transcriptional responses underlying microglia polarization by glioma.
Marta MaleszewskaAleksandra SterankaMagdalena SmiechBeata KazaPaulina PilancMichal DabrowskiBozena KaminskaPublished in: Glia (2020)
Microglia, resident myeloid cells of the central nervous system (CNS), act as immune sentinels that contribute to maintenance of physiological homeostasis and respond to any perturbation in CNS. Microglia could be polarized by various stimuli to perform dedicated functions and instigate inflammatory or pro-regenerative responses. Microglia and peripheral macrophages accumulate in glioblastomas (GBMs), malignant brain tumors, but instead of initiating antitumor responses, these cells are polarized to the pro-invasive and immunosuppressive phenotype which persists for a long time and contributes to a "cold" immune microenvironment of GBMs. Molecular mechanisms underlying this long-lasting "microglia memory" are unknown. We hypothesized that this state may rely on epigenetic silencing of inflammation-related genes. In this study, we show that cultured microglia pre-exposed to glioma-conditioned medium (GCM) acquire a "transcriptional memory" and display reduced expression of inflammatory genes after re-stimulation with lipopolysaccharide. Unstimulated microglia have unmethylated DNA and active histone marks at selected gene promoters indicating chromatin accessibility. Adding GCM increases expression and enzymatic activity of histone deacetylases (Hdac), leading to erasure of histone acetylation at tested genes. Later inflammatory genes acquire repressive histone marks (H3K27 trimethylation), which correlates with silencing of their expression. GCM induced genes acquire active histone marks. Hdac inhibitors block GCM-induced changes of histone modifications and restore microglia ability to initiate effective inflammatory responses. Altogether, we show a scenario of distinct histone modifications underlying polarization of microglia by glioma. We demonstrate contribution of epigenetic mechanisms to glioma-induced "transcriptional memory" in microglia resulting in the tumor-supportive phenotype.
Keyphrases
- dna methylation
- inflammatory response
- neuropathic pain
- genome wide
- gene expression
- oxidative stress
- poor prognosis
- transcription factor
- induced apoptosis
- high glucose
- diabetic rats
- stem cells
- genome wide identification
- lps induced
- dendritic cells
- endothelial cells
- toll like receptor
- long non coding rna
- immune response
- mesenchymal stem cells
- signaling pathway
- nitric oxide
- single molecule
- quality improvement
- genome wide analysis
- circulating tumor
- pi k akt
- heat shock protein