Selection and validation of reference gene for RT-qPCR studies in co-culture system of mouse cementoblasts and periodontal ligament cells.
Jiawen YongSabine GroegerGisela Ruiz-HeilandSabine RufPublished in: BMC research notes (2022)
The results demonstrated that PPIB, GUSB and RPLP0 turned out to be the three most stable reference genes for OCCM-30 in the co-culture system, while PPIB, POLR2A and RPLP0 have the three highest rankings for SV-PDL cells in the co-culture system. The most stable gene combination were PPIB and POLR2A in the co-culture system. In conclusion, PPIB is overall the most stably expressed reference gene for OCCM-30 or SV-PDL cell line in the system. The combination of PPIB and POLR2A as reference genes are indicated to be the potential and mandatory to obtain accurate quantification results for normalizing RT-qPCR data in genes of interest expression in these two cell lines co-culture systems.
Keyphrases
- genome wide identification
- genome wide
- induced apoptosis
- genome wide analysis
- copy number
- cell cycle arrest
- dna methylation
- transcription factor
- bioinformatics analysis
- poor prognosis
- high resolution
- signaling pathway
- oxidative stress
- gene expression
- cell proliferation
- risk assessment
- climate change
- electronic health record
- mass spectrometry
- clinical evaluation