Coronary Artery Disease and Aortic Valve Stenosis: A Urine Proteomics Study.
Luís PerpétuoAntónio S BarrosJéssica DalsucoRita Nogueira-FerreiraPedro Resende-GonçalvesInês Falcão-PiresRita FerreiraAdelino F Leite-MoreiraFábio TrindadeRui VitorinoPublished in: International journal of molecular sciences (2022)
Coronary artery disease (CAD) and the frequently coexisting aortic valve stenosis (AVS) are heart diseases accounting for most cardiac surgeries. These share many risk factors, such as age, diabetes, hypertension, or obesity, and similar pathogenesis, including endothelial disruption, lipid and immune cell infiltration, inflammation, fibrosis, and calcification. Unsuspected CAD and AVS are sometimes detected opportunistically through echocardiography, coronary angiography, and magnetic resonance. Routine biomarkers for early detection of either of these atherosclerotic-rooted conditions would be important to anticipate the diagnosis. With a noninvasive collection, urine is appealing for biomarker assessment. We conducted a shotgun proteomics exploratory analysis of urine from 12 CAD and/or AVS patients and 11 controls to identify putative candidates to differentiate these diseases from healthy subjects. Among the top 20 most dysregulated proteins, TIMP1, MMP2 and vWF stood out, being at least 2.5× increased in patients with CAD/AVS and holding a central position in a network of protein-protein interactions. Moreover, their assessment in an independent cohort (19 CAD/AVS and 10 controls) evidenced strong correlations between urinary TIMP1 and vWF levels and a common cardiovascular risk factor - HDL (r = 0.59, p < 0.05, and r = 0.64, p < 0.01, respectively).
Keyphrases
- coronary artery disease
- aortic valve
- aortic stenosis
- risk factors
- transcatheter aortic valve replacement
- aortic valve replacement
- transcatheter aortic valve implantation
- percutaneous coronary intervention
- magnetic resonance
- cardiovascular events
- coronary artery bypass grafting
- end stage renal disease
- type diabetes
- ejection fraction
- chronic kidney disease
- mass spectrometry
- left ventricular
- metabolic syndrome
- cardiovascular disease
- blood pressure
- newly diagnosed
- oxidative stress
- insulin resistance
- heart failure
- prognostic factors
- peritoneal dialysis
- atrial fibrillation
- fatty acid
- glycemic control