Single Nucleus RNA Sequencing of Remnant Kidney Biopsies and Urine Cell RNA Sequencing Reveal Cell Specific Markers of Covid-19 Acute Kidney Injury.
Reetika GhagMadhurima KaushalGerald NwanneAmanda KnotenKrzysztof KirylukAvi RosenbergSteve MenezSerena M BagnascoC John SperatiMohamed G AttaJoseph P GautJames C WilliamsTarek M El-AchkarLois J ArendChirag R ParikhSanjay JainPublished in: bioRxiv : the preprint server for biology (2023)
The manuscript describes innovation, application and discovery that impact clinical care in kidney disease. First, the approach to maximize use of remnant frozen clinical biopsies to inform on clinically relevant molecular features can augment existing pathological workflow for any frozen tissue without much change in the protocol. Second, this approach is transformational in medical crises such as pandemics where mechanistic insights are needed to evaluate organ injury, targets for drug therapy and diagnostic and prognostic markers. Third, the cell type specific and soluble markers identified and validated can be used for diagnoses or prognoses in AKI due to different etiologies and in multiorgan injury.
Keyphrases
- single cell
- acute kidney injury
- healthcare
- high throughput
- cell therapy
- coronavirus disease
- sars cov
- randomized controlled trial
- cardiac surgery
- palliative care
- small molecule
- stem cells
- ultrasound guided
- quality improvement
- emergency department
- electronic health record
- pain management
- respiratory syndrome coronavirus