Baicalein Inhibits Orthotopic Human Non-Small Cell Lung Cancer Xenografts via Src/Id1 Pathway.
Zhengxiao ZhaoBaojun LiuJing SunLinwei LuLumei LiuJian QiuQiuping LiChen YanShan JiangNabijan MohammadtursunWenjuan MaMihui LiJing-Cheng DongWeiyi GongPublished in: Evidence-based complementary and alternative medicine : eCAM (2019)
Non-small cell lung cancer (NSCLC) is one of the most lethal cancers worldwide. Inhibitor of differentiation 1 (Id1) is the member mostly linked to tumorigenesis in Id family and a potential molecular target in cancer therapy. In the current study, we established an orthotopic lung cancer model by injecting athymic nude mice with A549 cells and evaluated the antitumor effect of baicalein and expression of Id1-related proteins in vivo and in vitro. Micro-CT images showed that tumor volume in baicalein group was significantly reduced. Western blot analysis revealed that baicalein suppressed the expression of Id1 protein, epithelial-to-mesenchymal transition (EMT) related molecules (N-Cadherin, vimentin), and angiogenesis related protein (VEGF-A), accompanied by upregulation of epithelial markers (such as E-cadherin). In addition, phosphorylation of upstream molecular Src was significantly restrained after baicalein treatment. This study firstly demonstrates that baicalein inhibits tumor growth in orthotopic human NSCLC xenografts via targeting Src/Id1 pathway.
Keyphrases
- endothelial cells
- poor prognosis
- cancer therapy
- small cell lung cancer
- tyrosine kinase
- vascular endothelial growth factor
- advanced non small cell lung cancer
- induced apoptosis
- deep learning
- binding protein
- cell proliferation
- long non coding rna
- computed tomography
- epithelial mesenchymal transition
- signaling pathway
- convolutional neural network
- risk assessment
- oxidative stress
- machine learning
- cell death
- climate change
- young adults
- skeletal muscle
- smoking cessation
- pet ct
- protein protein