Aberrant B cell activation and B cell subpopulations in rheumatoid arthritis: analysis by clinical activity, autoantibody seropositivity, and treatment.
José Javier Morales-NúñezJosé Francisco Muñoz-ValleMariel García-ChagollánSergio Cerpa CruzGloria Esther Martínez BonillaVianey Monserrat Medina RosalesSaúl Alberto Díaz PérezFerdinando NicolettiJorge Hernández-BelloPublished in: Clinical and experimental immunology (2023)
Few studies analyze the role of B cells subpopulations in rheumatoid arthritis (RA) pathophysiology. Therefore, this study aimed to analyze the differences in B cell subpopulations and B cell activation according to disease activity, RA subtype, and absence of DMARDs therapy. These subgroups were compared with control subjects (CS). 139 subjects were included, of which 114 were RA patients and 25 were controls. Patients were divided into 99 with seropositive RA, six with seronegative RA, and nine without DMARDs. The patients with seropositive RA were subclassified based on the DAS28 index. A 7-color multicolor flow cytometry panel was used to identify B-cell immunophenotypes and cell activation markers. There were no changes in total B cells frequencies between RA patients and controls. However, a lower frequency of memory B cells and pre-plasmablasts was observed in seropositive RA compared to controls (p <0.0001; p =0.0043, respectively). In contrast, a higher frequency of mature B cells was observed in RA than in controls (p =0.0002). Among patients with RA, those with moderate activity had a higher percentage of B cells (p =0.0021). The CD69 + marker was increased (p <0.0001) in RA compared to controls, while the CD40 + frequency was decreased in patients (p <0.0001). Transitional, naïve, and double-negative B cell subpopulations were higher in seronegative RA than in seropositive (p <0.01). In conclusion, seropositive and seronegative RA patients, there are alterations in B cell activation and B cell subpopulations, independently of clinical activity and DMARDs therapy.
Keyphrases
- rheumatoid arthritis
- disease activity
- end stage renal disease
- ankylosing spondylitis
- rheumatoid arthritis patients
- systemic lupus erythematosus
- ejection fraction
- newly diagnosed
- peritoneal dialysis
- prognostic factors
- stem cells
- magnetic resonance imaging
- bone marrow
- computed tomography
- mesenchymal stem cells
- patient reported outcomes
- cell therapy