The roles of plasma accessible and cytosolic carbonic anhydrases in bicarbonate (HCO₃^-) excretion in Pacific hagfish (Eptatretus stoutii).

Pacific hagfish (Eptatretus stoutii) are marine scavengers and feed on decaying animal carrion by burrowing their bodies inside rotten carcasses where they are exposed to several threatening environmental stressors, including hypercapnia (high partial pressures of CO₂). Hagfish possess a remarkable capacity to tolerate hypercapnia, and their ability to recover from acid-base disturbances is well known. To deal with the metabolic acidosis resulting from exposure to high CO₂, hagfish can mount a rapid elevation of plasma HCO₃^- concentration (hypercarbia). Once PCO₂ is restored, hagfish quickly excrete their HCO₃^- load, a process that likely involves the enzyme carbonic anhydrase (CA), which catalyzes HCO₃^- dehydration into CO₂ at the hagfish gills. We aimed to characterize the role of branchial CA in CO₂/HCO₃^- clearance from the plasma at the gills of E. stoutii, under control and high PCO₂ (hypercapnic) exposure conditions. We assessed the relative contributions of plasma accessible versus intracellular (cytosolic) CA to gill HCO₃^- excretion by measuring in situ [¹⁴C]-HCO₃^- fluxes. To accomplish this, we employed a novel surgical technique of individual gill pouch arterial perfusion combined with perifusion of the gill afferent to efferent water ducts. [¹⁴C]-HCO₃^- efflux was measured at the gills of fish exposed to control, hypercapnic (48 h) and recovery from hypercapnia conditions (6 h), in the presence of two well-known pharmacological inhibitors of CA, the membrane impermeant C18 (targets membrane bound, plasma accessible CA) and membrane-permeant acetazolamide, which targets all forms of CA, including extracellular and intracellular cytosolic CAs. C18 did not affect HCO₃^- flux in control fish, whereas acetazolamide resulted in a significant reduction of 72%. In hypercapnic fish, HCO₃^- fluxes were much higher and perfusion with acetazolamide caused a reduction of HCO₃^- flux by 38%. The same pattern was observed for fish in recovery, where in all three experimental conditions, there was no significant inhibition of plasma-accessible CA. We also observed no change in CA enzyme activity (measured in vitro) in any of the experimental PCO₂ conditions. In summary, our data suggests that there are additional pathways for HCO₃^- excretion at the gills of hagfish that are independent of plasma-accessible CA.