Temperature-Dependent Dynamics of Giant Vesicles Composed of Hydrolysable Lipids Having an Amide Linkage.
Maito TameyukiHisato HiranakaTaro ToyotaKouichi AsakuraTaisuke BannoPublished in: Langmuir : the ACS journal of surfaces and colloids (2019)
Various amphiphiles including surfactants and lipids have been designed and synthesized to improve and create new functionalities. In particular, the emergence of cell-like behaviors of giant vesicles (GVs) composed of synthetic lipids has drawn much attention in the development of chemical models for cells. The aim of this study was to measure temperature-dependent morphological changes of GVs induced by fragmentation and subsequent growth using hydrolysable cationic lipids having an amide linkage. Results from differential scanning calorimetry, fluorescence spectroscopy using an environment-responsive probe, and confocal Raman microscopy showed that the dynamics observed were due to changes in the vesicle membrane, including variation in the lipid composition, induced by thermal stimulation.
Keyphrases
- single molecule
- fatty acid
- high resolution
- induced apoptosis
- genome wide
- optical coherence tomography
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- gene expression
- oxidative stress
- stem cells
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- label free
- hepatitis c virus
- dna methylation
- mesenchymal stem cells
- electron microscopy
- hiv infected
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