αEβ7, α4β7 and α4β1 integrin contributions to T cell distribution in blood, cervix and rectal tissues: Potential implications for HIV transmission.
Catia T PercianiWalter JaokoBashir FarahMario A OstrowskiOmu AnzalaKelly S MacDonaldnull nullPublished in: PloS one (2018)
Cell surface expression of α4β7, α4β1 and αEβ7 integrins play a key role in T cell distribution. Understanding the contribution of integrins to the density and ratios of CD4+: CD4negT cell at the portals of entry for HIV is of fundamental importance for the advance of more effective HIV prevention strategies. We therefore set out to characterize and compare the expression of α4β7, α4β1 and αEβ7 integrins on systemic, cervical and rectal CD4+ and CD4negT cells isolated from a cohort of healthy Kenyan women at low risk for sexually transmitted infections (STI) (n = 45). Here we show that blood and cervix were enriched in α4+β1+CD4+T cells and α4+β7hiCD4+T cells, whereas the rectum had an equal frequency of α4+β7hiCD4+T cells and αE+β7hiCD4+T cells. Most cervical and rectal αE+β7hiCD4+T cells expressed CCR5 as well as CD69. Interestingly, αEβ7 was the predominant integrin expressed by CD4negT cells in both mucosal sites, outnumbering αE+β7hiCD4+T cells approximately 2-fold in the cervix and 7-fold in the rectum. The majority of αE+β7hiCD4negT cells expressed CD69 at the mucosa. Taken together, our results show unique tissue-specific patterns of integrin expression. These results can help in guiding vaccine design and also the use of therapeutically targeting integrin adhesion as a means to preventing HIV.
Keyphrases
- induced apoptosis
- poor prognosis
- hiv positive
- antiretroviral therapy
- cell cycle arrest
- hiv testing
- human immunodeficiency virus
- hiv infected
- hepatitis c virus
- men who have sex with men
- nk cells
- hiv aids
- rectal cancer
- preterm birth
- cell surface
- gene expression
- endoplasmic reticulum stress
- type diabetes
- escherichia coli
- cell adhesion
- single cell
- cell migration
- risk assessment
- signaling pathway
- staphylococcus aureus
- immune response
- long non coding rna
- pregnant women
- cell therapy
- biofilm formation
- adipose tissue