Metronidazole Delivery Nanosystem Able To Reduce the Pathogenicity of Bacteria in Colorectal Infection.
Ana OliveiraAna AraújoLuísa C RodriguesCatarina S SilvaRui L ReisNuno M NevesPedro LeãoAlbino MartinsPublished in: Biomacromolecules (2022)
Metronidazole (MTZ) is a drug potentially used for the treatment of intestinal infections, namely, the ones caused by colorectal surgery. The traditional routes of administration decrease its local effectiveness and present off-site effects. To circumvent such limitations, herein a drug delivery system (DDS) based on MTZ-loaded nanoparticles (NPs) immobilized at the surface of electrospun fibrous meshes is proposed. MTZ at different concentrations (1, 2, 5, and 10 mg mL -1 ) was loaded into chitosan-sodium tripolyphosphate NPs. The MTZ loaded into NPs at the highest concentration showed a quick release in the first 12 h, followed by a gradual release. This DDS was not toxic to human colonic cells. When tested against different bacterial strains, a significant reduction of Escherichia coli and Staphylococcus aureus was observed, but no effect was found against Enterococcus faecalis . Therefore, this DDS offers high potential to locally prevent the occurrence of infections after colorectal anastomosis.
Keyphrases
- drug delivery
- wound healing
- escherichia coli
- staphylococcus aureus
- cancer therapy
- biofilm formation
- induced apoptosis
- endothelial cells
- randomized controlled trial
- oxide nanoparticles
- risk assessment
- systematic review
- cell cycle arrest
- oxidative stress
- signaling pathway
- induced pluripotent stem cells
- replacement therapy
- endoplasmic reticulum stress
- cell death
- pseudomonas aeruginosa
- candida albicans