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Cytokine-inducible promoters to drive dynamic transgene expression: The "Smart Graft" strategy.

Andrea FischerKatrin ManskeJochen SeisslerDirk WohlleberNina SimmLelia Wolf-van BuerckPercy KnolleAngelika SchniekeKonrad Fischer
Published in: Xenotransplantation (2020)
This is the first in vivo comparison of existing and newly designed cytokine-inducible promoters. Optimization of promoter structure resulted in almost 10-fold inducibility of promoter activity. Such a rapid and dynamically regulated response to inflammation and cell damage could reduce initial graft rejection, making the "Smart Graft" approach a useful means of modulating the expression of immune regulatory transgenes to avoid deleterious effects on porcine and human health. Expressing transgenes in this fashion could provide a safer organ for transplantation.
Keyphrases
  • human health
  • transcription factor
  • poor prognosis
  • risk assessment
  • dna methylation
  • oxidative stress
  • gene expression
  • cell therapy
  • climate change
  • single cell
  • signaling pathway
  • stem cells
  • bone marrow