NDV-3A vaccination prevents C. albicans colonization of jugular vein catheters in mice.
Abdullah AlqarihiShakti SinghJohn E EdwardsAshraf S IbrahimPriya UppuluriPublished in: Scientific reports (2019)
NDV-3A, a novel fungal vaccine undergoing clinical trials, contains a recombinant version of the Candida albicans rAls3 N-terminus protein (rAls3p-N) in aluminum hydroxide. In a Phase 1b/2a clinical trial, NDV-3A protected women from recurrent vulvovaginal candidiasis. Here, we reveal that active immunization in mice with NDV-3A induces high titers of anti-rAls3p-N antibodies that interfere with C. albicans ability to adhere to and invade endothelial cells, and form biofilm in vitro. Anti-rAls3p-N antibodies also significantly inhibit yeast dispersal from the hyphal layers of biofilms. Compared to placebo, NDV-3A vaccination inhibited C. albicans dissemination to kidneys and prevented colonization of central venous catheters in mice. Overall, these preclinical studies suggest that NDV-3A may serve as an immunotherapeutic strategy for prevention of infections on indwelling medical devices.
Keyphrases
- candida albicans
- clinical trial
- biofilm formation
- high fat diet induced
- endothelial cells
- double blind
- phase ii
- phase iii
- wild type
- randomized controlled trial
- single cell
- metabolic syndrome
- gene expression
- escherichia coli
- pseudomonas aeruginosa
- psychometric properties
- adipose tissue
- vascular endothelial growth factor
- breast cancer risk