Mechanical and molecular parameters that influence the tendon differentiation potential of C3H10T1/2 cells in 2D- and 3D-culture systems.
Ludovic GautMarie-Ange BonninCédrine BlavetIsabelle CacciapuotiMonika OrpelMathias MericskayDelphine DuprezPublished in: Biology open (2020)
One of the main challenges relating to tendons is to understand the regulators of the tendon differentiation program. The optimum culture conditions that favor tendon cell differentiation have not been identified. Mesenchymal stem cells present the ability to differentiate into multiple lineages in cultures under different cues ranging from chemical treatment to physical constraints. We analyzed the tendon differentiation potential of C3H10T1/2 cells, a murine cell line of mesenchymal stem cells, upon different 2D- and 3D-culture conditions. We observed that C3H10T1/2 cells cultured in 2D conditions on silicone substrate were more prone to tendon differentiation, assessed with the expression of the tendon markers Scx, Col1a1 and Tnmd as compared to cells cultured on plastic substrate. The 3D-fibrin environment was more favorable for Scx and Col1a1 expression compared to 2D cultures. We also identified TGFβ2 as a negative regulator of Tnmd expression in C3H10T1/2 cells in 2D and 3D cultures. Altogether, our results provide us with a better understanding of the culture conditions that promote tendon gene expression and identify mechanical and molecular parameters upon which we could act to define the optimum culture conditions that favor tenogenic differentiation in mesenchymal stem cells.
Keyphrases
- mesenchymal stem cells
- anterior cruciate ligament reconstruction
- rotator cuff
- poor prognosis
- gene expression
- umbilical cord
- bone marrow
- endothelial cells
- transcription factor
- long non coding rna
- mental health
- dna methylation
- cell therapy
- risk assessment
- quality improvement
- oxidative stress
- signaling pathway
- human health
- combination therapy
- amino acid