DNA origami demonstrate the unique stimulatory power of single pMHCs as T cell antigens.
Joschka HellmeierRene PlatzerAlexandra S EklundThomas SchlichthaerleAndreas KarnerViktoria MotschMagdalena C SchneiderElke KurzVictor BamiehMario BrameshuberJohannes PreinerRalf JungmannHannes StockingerGerhard J SchützJohannes B HuppaEva SevcsikPublished in: Proceedings of the National Academy of Sciences of the United States of America (2021)
T cells detect with their T cell antigen receptors (TCRs) the presence of rare agonist peptide/MHC complexes (pMHCs) on the surface of antigen-presenting cells (APCs). How extracellular ligand binding triggers intracellular signaling is poorly understood, yet spatial antigen arrangement on the APC surface has been suggested to be a critical factor. To examine this, we engineered a biomimetic interface based on laterally mobile functionalized DNA origami platforms, which allow for nanoscale control over ligand distances without interfering with the cell-intrinsic dynamics of receptor clustering. When targeting TCRs via stably binding monovalent antibody fragments, we found the minimum signaling unit promoting efficient T cell activation to consist of two antibody-ligated TCRs within a distance of 20 nm. In contrast, transiently engaging antigenic pMHCs stimulated T cells robustly as well-isolated entities. These results identify pairs of antibody-bound TCRs as minimal receptor entities for effective TCR triggering yet validate the exceptional stimulatory potency of single isolated pMHC molecules.
Keyphrases
- circulating tumor
- single cell
- induced apoptosis
- single molecule
- cell free
- magnetic resonance
- cell cycle arrest
- binding protein
- cell therapy
- regulatory t cells
- photodynamic therapy
- rna seq
- quantum dots
- dendritic cells
- drug delivery
- computed tomography
- mesenchymal stem cells
- stem cells
- immune response
- signaling pathway
- contrast enhanced
- cell proliferation
- molecularly imprinted
- reactive oxygen species
- nucleic acid
- bone marrow
- mass spectrometry
- pi k akt