Expression/Activation of PAR-1 in Airway Epithelial Cells of COPD Patients: Ex Vivo/In Vitro Study.
Angela Marina MontalbanoGiuseppina ChiapparaGiusy Daniela AlbanoMaria FerraroCaterina Di SanoPatrizio VituloLoredana PipitoneFabio Luigi Massimo RicciardoloGiulia AnzaloneMirella ProfitaPublished in: International journal of molecular sciences (2021)
The role of PAR-1 expression and activation was described in epithelial cells from the central and distal airways of COPD patients using an ex vivo/in vitro model. PAR-1 immunoreactivity was studied in epithelial cells from surgical specimens of the central and distal airways of COPD patients and healthy control (HC). Furthermore, PAR-1 expression and activation were measured in both the human bronchial epithelial cell line (16HBE) and normal human bronchial epithelial cells (NHBEs) exposed to cigarette smoke extract (CSE) (10%) or thrombin. Finally, cell proliferation, apoptosis, and IL-8 release were detected in stimulated NHBEs. We identified higher levels of PAR-1 expression/activation in epithelial cells from the central airways of COPD patients than in HC. Active PAR-1 increased in epithelial cells from central and distal airways of COPD, with higher levels in COPD smokers (correlated with pack-years) than in COPD ex-smokers. 16HBE and NHBEs exposed to CSE or thrombin showed increased levels of active PAR-1 (localized in the cytoplasm) than baseline conditions, while NHBEs treated with thrombin or CSE showed increased levels of IL-8 proteins, with an additional effect when used in combination. Smoking habits generate the upregulation of PAR-1 expression/activation in airway epithelial cells, and promoting IL-8 release might affect the recruitment of infiltrating cells in the airways of COPD patients.
Keyphrases
- end stage renal disease
- chronic obstructive pulmonary disease
- ejection fraction
- newly diagnosed
- poor prognosis
- cell proliferation
- chronic kidney disease
- cystic fibrosis
- prognostic factors
- peritoneal dialysis
- endothelial cells
- patient reported outcomes
- minimally invasive
- endoplasmic reticulum stress
- long non coding rna
- cell death
- patient reported
- fine needle aspiration