Caffeic acid improves intestinal barrier functions by regulating colonic bacteria and tight junction protein expression and alleviating inflammation in weaning piglets.

The experiment investigated the effect of caffeic acid on bacteria, short-chain fatty acids (SCFA), and the expression of tight junction protein and inflammation related genes in the colon of weaning piglets. Thirty-six weaning piglets were allocated to three treatment groups, which were fed with a basal diet, a basal diet supplemented with 250 mg/kg or 500 mg/kg caffeic acid for 28 days. The results showed that caffeic acid treatment increased the contents of acetate acid, propionate acid and total SCFA. Moreover, real-time quantitative PCR showed that the number of Bifidobacterium ( p  < 0.05) and Lactobacillus ( p  < 0.05) were increased and the number of Escherichia coli ( p  < 0.05) was decreased by caffeic acid in colonic mucosa. Real-time quantitative PCR also showed that the mRNA levels of zonula occludens-1 ( p  < 0.01), claudin-1 ( p  < 0.01), occludin ( p  < 0.01), mucin 1 ( MUC1 ) ( p  < 0.01), MUC2 ( p  < 0.01), interleukin 4 (IL-4) ( p  < 0.01) and IL-10 ( p  < 0.05) were increased, while the mRNA expression levels of histone deacetylases ( p  < 0.01), IL-1 ( p  < 0.01), IL-6 ( p  < 0.01) and tumor necrosis factor-α ( TNF-α ) ( p  < 0.01) were decreased, by caffeic acid in colonic mucosa. These results suggested that caffeic acid could improve intestinal barrier function in weaned pigs, which might be mediated by regulating colonic bacteria and tight junction protein expression and alleviating inflammation.