4-Hydroxynonenal and 4-Oxononenal Differentially Bind to the Redox Sensor MitoNEET.
Dayna ArnettAlexandria QuillinWerner J GeldenhuysMichael A MenzeMary E KonklePublished in: Chemical research in toxicology (2019)
MitoNEET is a CDGSH iron-sulfur protein that has been a target for drug development for diseases such as type-2 diabetes, cancer, and Parkinson's disease. Functions proposed for mitoNEET are as a redox sensor and regulator of free iron in the mitochondria. We have investigated the reactivity of mitoNEET toward the reactive electrophiles 4-hydroxynonenal (HNE) and 4-oxononenal (ONE) that are produced from the oxidation of polyunsaturated fatty acid during oxidative stress. Proteomic, electrophoretic, and spectroscopic analysis has shown that HNE and ONE react in a sequence selective manner that was unexpected considering the structure similarity of these two reactive electrophiles.
Keyphrases
- fatty acid
- type diabetes
- oxidative stress
- papillary thyroid
- molecular docking
- dna damage
- electron transfer
- iron deficiency
- hydrogen peroxide
- amino acid
- cardiovascular disease
- transcription factor
- squamous cell
- squamous cell carcinoma
- insulin resistance
- protein protein
- atomic force microscopy
- reactive oxygen species
- adipose tissue
- nitric oxide
- lymph node metastasis
- young adults
- small molecule
- heat shock
- high resolution