Native-like SARS-CoV-2 Spike Glycoprotein Expressed by ChAdOx1 nCoV-19/AZD1222 Vaccine.
Yasunori WatanabeLuiza MendonçaElizabeth R AllenAndrew HoweMercede LeeJoel D AllenHimanshi ChawlaDavid PulidoFrancesca DonnellanHannah DaviesMarta UlaszewskaSandra Belij-RammerstorferSusan MorrisAnna-Sophia KrebsWanwisa DejnirattisaiJuthathip MongkolsapayaPiyada SupasaGavin R ScreatonCatherine M GreenTeresa LambePeijun ZhangSarah C GilbertMax CrispinPublished in: ACS central science (2021)
Vaccine development against the SARS-CoV-2 virus focuses on the principal target of the neutralizing immune response, the spike (S) glycoprotein. Adenovirus-vectored vaccines offer an effective platform for the delivery of viral antigen, but it is important for the generation of neutralizing antibodies that they produce appropriately processed and assembled viral antigen that mimics that observed on the SARS-CoV-2 virus. Here, we describe the structure, conformation, and glycosylation of the S protein derived from the adenovirus-vectored ChAdOx1 nCoV-19/AZD1222 vaccine. We demonstrate native-like post-translational processing and assembly, and reveal the expression of S proteins on the surface of cells adopting the trimeric prefusion conformation. The data presented here confirm the use of ChAdOx1 adenovirus vectors as a leading platform technology for SARS-CoV-2 vaccines.
Keyphrases
- sars cov
- respiratory syndrome coronavirus
- immune response
- gene therapy
- poor prognosis
- induced apoptosis
- high throughput
- binding protein
- molecular dynamics simulations
- electronic health record
- cell cycle arrest
- gene expression
- cell proliferation
- dendritic cells
- machine learning
- small molecule
- long non coding rna
- inflammatory response
- cell death
- endoplasmic reticulum stress