TUG1 long non-coding RNA enlists the USF1 transcription factor to overexpress ROMO1 leading to hepatocellular carcinoma growth and metastasis.
Shihai LiuJing QiuWeitai HeChao GengGuifang HeChangchang LiuDuo CaiXiangping LiuBen TianHuazheng PanPublished in: MedComm (2020)
Hepatocellular carcinoma (HCC) is a prevalent and highly aggressive cancer. Long non-coding RNAs (lncRNAs) are recognized as potential molecular targets for HCC and are currently under increased research focus. Here, we investigate the regulatory processes underlying the axis of the lncRNA taurine upregulated gene 1 (TUG1), Upstream Transcription Factor 1 (USF1), and reactive oxygen species modulator 1 (ROMO1) in the propagation and metastasis of HCC cells. Distribution of lncRNA TUG1 was found to be prominent in HCC cell cytoplasm and nuclei. LncRNA TUG1 conscripted the USF1 transcription factor to enhance the promoter function of ROMO1. Enlisting the USF1 transcription factor to increase ROMO1 expression following upregulation of TUG1 lncRNA enhanced HCC Huh7 cell proliferation, motility, and metastasis. Rapid tumor proliferation in nude mice provided in vivo verification. The importance of the lncRNA TUG1/USF1/ROMO1 complex as a target for HCC therapy is a key result of this investigation which is exemplified by its role in regulating the proliferation, motility, and metastasis of HCC cells.
Keyphrases
- long non coding rna
- transcription factor
- poor prognosis
- genome wide identification
- induced apoptosis
- cell proliferation
- dna binding
- signaling pathway
- reactive oxygen species
- cell cycle arrest
- dna methylation
- oxidative stress
- biofilm formation
- gene expression
- single cell
- adipose tissue
- endoplasmic reticulum stress
- smoking cessation
- insulin resistance
- risk assessment
- type diabetes
- escherichia coli
- candida albicans
- bone marrow
- cell cycle
- cystic fibrosis
- sensitive detection
- single molecule