Novel method utilizing bisulfite conversion with dual amplification-refractory mutation system polymerase chain reaction to detect circulating pancreatic β-cell cfDNA.
Asami OkadaMisuzu Yamada-YamashitaYukari TominagaKyoka JoHiroyasu MoriReiko SuzukiMasashi IshizuMotoyuki TamakiYuko AkehiYuichi TakashiDaisuke KogaEisuke ShimokitaFuminori TaniharaKiyoe KurahashiSumiko YoshidaYukari MitsuiShiho MasudaItsuro EndoKen-Ichi AiharaShoji KagamiMasahiro AbeKevin FerreriYoshio FujitaniMunehide MatsuhisaAkio KurodaPublished in: Journal of diabetes investigation (2022)
We developed a novel method for detecting unmethylated insulin DNA in circulation that can be performed using a conventional real-time PCR system. This method would be useful for analyzing dynamic profiles of β-cells in human disease such as type 1 diabetes.
Keyphrases
- type diabetes
- real time pcr
- endothelial cells
- induced apoptosis
- cardiovascular disease
- nucleic acid
- cell cycle arrest
- cell therapy
- stem cells
- oxidative stress
- metabolic syndrome
- single molecule
- cell free
- induced pluripotent stem cells
- cell death
- skeletal muscle
- circulating tumor
- signaling pathway
- adipose tissue
- cell proliferation
- endoplasmic reticulum stress
- circulating tumor cells
- pluripotent stem cells