A hydrogen-driven biocatalytic approach to recycling synthetic analogues of NAD(P)H.

We demonstrate a recycling system for synthetic nicotinamide cofactor analogues using a soluble hydrogenase with turnover number of >1000 for reduction of the cofactor analogues by H 2 . Coupling this system to an ene reductase, we show quantitative conversion of N -ethylmaleimide to N -ethylsuccinimide. The biocatalyst system retained >50% activity after 7 h.