Exploring new potential role of DDB2 by host cell reactivation assay in human tumorigenic cells.

Elisabetta BassiPaola PeruccaIsabella GuardamagnaEnnio ProsperiLucia A StivalaOrnella Cazzalini

Published in: BMC cancer (2019)

The HCR confirms itself as a very helpful approach to assess in the cellular context the effect of expressing mutant vs Wt NER proteins on the DNA damage response. Loss of interaction of DDB2 and PCNA affects negatively DNA repair efficiency.

Keyphrases

dna repair
dna damage response
dna damage
induced apoptosis
endothelial cells
cell cycle arrest
single cell
high throughput
induced pluripotent stem cells
cell therapy
wild type
pluripotent stem cells
oxidative stress
signaling pathway
bone marrow