C 20 - nor -Abietane and Three Abietane Diterpenoids from Plectranthus mutabilis Leaves as P-Glycoprotein Modulators.
Epole N NtungweSofija Jovanović StojanovNoélia M DuarteNuno R CandeiasAna M Díaz-LanzaMáté VágvölgyiAttila HunyadiMilica PesicPatrícia RíjoPublished in: ACS medicinal chemistry letters (2022)
In this study, a bioguided fractionation of Plectranthus mutabilis extract was performed by chromatographic methods. It yielded one new nor -abietane diterpene, mutabilol ( 1 ), and three known abietanes, coleon-U-quinone ( 2 ), 8α,9α-epoxycoleon-U-quinone ( 3 ), and coleon U ( 4 ). The abietane diterpenoid 5 was also tentatively identified using HPLC-MS/MS. Moreover, the extract profile and quantification of each isolated compound were determined by HPLC-DAD. Compound 4 was the major compound in the extract. Compounds 2 - 4 were found to be selective toward cancer cell lines and were able to inhibit P-glycoprotein (P-gp) activity in NCI-H460/R cells at longer exposure of 72 h and consequently revert doxorubicin (DOX) resistance in subsequent combined treatment. None of the compounds influenced the P-gp expression in NCI-H460/R cells, while the extract significantly increased it.
Keyphrases
- ms ms
- simultaneous determination
- induced apoptosis
- oxidative stress
- cell cycle arrest
- liquid chromatography tandem mass spectrometry
- high performance liquid chromatography
- anti inflammatory
- poor prognosis
- small molecule
- mass spectrometry
- squamous cell carcinoma
- drug delivery
- signaling pathway
- papillary thyroid
- endoplasmic reticulum stress
- tandem mass spectrometry
- ultra high performance liquid chromatography
- cancer therapy
- combination therapy
- young adults