HrgS (Avin_34990), a novel histidine-kinase related to GacS, regulates alginate synthesis in Azotobacter vinelandii.
Liliana López-PliegoVerónica González-AcocalDiana Laura García-GonzálezJimena Itzel Reyes-NicolauZaira Sánchez-CuapioAlan Shared Meneses-CarbajalLuis Ernesto Fuentes-RamírezMiguel CastañedaPublished in: FEMS microbiology letters (2022)
Azotobacter vinelandii is a soil bacterium that produces alginates, a family of polymers of biotechnological interest. In A. vinelandii, alginate production is controlled by the two-component system GacS/GacA. GacS/GacA, in turn, regulates the Rsm post-transcriptional regulatory system establishing a cascade that regulates alginate biosynthesis by controlling the expression of the algD biosynthetic gene. In Pseudomonas aeruginosa, GacS/GacA is influenced by other histidine-kinases constituting a multicomponent signal transduction system. In this study, we explore the presence of GacS-related histidine-kinases in A. vinelandii and discover a novel histidine-kinase (Avin_34990, renamed HrgS). This histidin-kinase acts as a negative regulator of alginate synthesis by controlling the transcription of the sRNAs belonging to the Rsm post-transcriptional regulatory system, for which a functional GacS is required.
Keyphrases
- transcription factor
- pseudomonas aeruginosa
- protein kinase
- tyrosine kinase
- gene expression
- poor prognosis
- cystic fibrosis
- genome wide
- sensitive detection
- oxidative stress
- living cells
- fluorescent probe
- multidrug resistant
- heat shock
- drug resistant
- acinetobacter baumannii
- long non coding rna
- quantum dots
- single molecule
- genome wide analysis