An intrinsically semi-permeable PDMS nanosheet encapsulating adipose tissue-derived stem cells for enhanced angiogenesis.
Megumi TakumaHajime FujitaNanami ZushiHisato NaganoRyuichi AzumaTomoharu KiyosawaToshinori FujiePublished in: Biomaterials science (2024)
Cell encapsulation devices are expected to be promising tools that can control the release of therapeutic proteins secreted from transplanted cells. The protein permeability of the device membrane is important because it allows the isolation of transplanted cells while enabling the effectiveness of the device. In this study, we investigated free-standing polymeric ultra-thin films (nanosheets) as an intrinsically semi-permeable membrane made from polydimethylsiloxane (PDMS). The PDMS nanosheet with a thickness of 600 nm showed intrinsic protein permeability, and the device fabricated with the PDMS nanosheet showed that VEGF secreted from implanted adipose tissue-derived stem cells (ASCs) could be released for at least 5 days. The ASC encapsulation device promoted angiogenesis and the development of granulation tissue 1 week after transplantation to the subcutaneous area of a mouse. This cell encapsulation device consisting of PDMS nanosheets provides a new method for pre-vascularization of the subcutaneous area in cell transplantation therapy.
Keyphrases
- cell therapy
- stem cells
- adipose tissue
- endothelial cells
- induced apoptosis
- single cell
- vascular endothelial growth factor
- randomized controlled trial
- systematic review
- insulin resistance
- drug delivery
- high resolution
- endoplasmic reticulum stress
- binding protein
- skeletal muscle
- amino acid
- quantum dots
- cell proliferation
- metabolic syndrome
- reduced graphene oxide
- mass spectrometry
- signaling pathway
- optical coherence tomography
- oxidative stress
- highly efficient
- protein protein
- single molecule
- smoking cessation