Antioxidant Effects of Walnut (Juglans regia L.) Kernel and Walnut Septum Extract in a D-Galactose-Induced Aging Model and in Naturally Aged Rats.
Marius Emil RusuCarmen GeorgiuAnca PopAndrei MocanBéla KissOliviu VostinaruIonel FizesanMaria-Georgia StefanAna-Maria GheldiuLetitia MatesRebeca MoldovanDana-Maria MunteanFelicia LoghinLaurian VlaseDaniela-Saveta PopaPublished in: Antioxidants (Basel, Switzerland) (2020)
Antioxidant dietary intervention is considered a potential strategy in delaying age-related dysfunctions. In this study of 56 days, we assessed the antioxidant effects of walnut kernel (WK) and walnut septum extract (WSE) in a D-galactose (D-gal)-induced aging model and in a naturally aged rat model. Young Wistar rats, treated with D-gal (1200 mg/week), and old rats received daily WK or WSE added to the feed. After 8 weeks, blood, liver, and brain samples were collected and hematological, biochemical, oxidative stress biomarkers, histological, and immunohistochemical analyses were performed. Moreover, acetylcholinesterase activity was investigated in brain homogenates. The outcomes demonstrated significant improvement in cellular antioxidant activity and/or decrease of reactive oxygen species, advanced glycation end products, nitric oxide, malondialdehyde, or increase of glutathione after WK or WSE intake in both models. Additionally, WSE showed hypoglycemic effect, and both WK and WSE lowered acetylcholinesterase activity. Both diets could protect neurons against the induced senescence and could reverse the pathological conditions in the physiological aged brain. Thus, dietary supplementation with WK or WSE can maintain the liver and brain health and reduce the risk of age-related diseases, as well as delaying the onset of aging processes.
Keyphrases
- oxidative stress
- diabetic rats
- resting state
- nitric oxide
- high glucose
- white matter
- anti inflammatory
- dna damage
- functional connectivity
- reactive oxygen species
- cerebral ischemia
- randomized controlled trial
- ischemia reperfusion injury
- healthcare
- induced apoptosis
- clinical trial
- mental health
- type diabetes
- endothelial cells
- drug induced
- brain injury
- spinal cord injury
- mass spectrometry
- subarachnoid hemorrhage
- insulin resistance
- body mass index
- hydrogen peroxide
- risk assessment
- adipose tissue
- human health
- social media
- nitric oxide synthase
- blood brain barrier
- health promotion