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Optogenetic control of RNA function and metabolism using engineered light-switchable RNA-binding proteins.

Renmei LiuJing YangJing YaoZhou ZhaoWei HeNi SuZeyi ZhangChenxia ZhangZhuo ZhangHaibo CaiLinyong ZhuYu-Zheng ZhaoShu QuanXianjun ChenYi Yang
Published in: Nature biotechnology (2022)
RNA-binding proteins (RBPs) play an essential role in regulating the function of RNAs in a cellular context, but our ability to control RBP activity in time and space is limited. Here, we describe the engineering of LicV, a photoswitchable RBP that binds to a specific RNA sequence in response to blue light irradiation. When fused to various RNA effectors, LicV allows for optogenetic control of RNA localization, splicing, translation and stability in cell culture. Furthermore, LicV-assisted CRISPR-Cas systems allow for efficient and tunable photoswitchable regulation of transcription and genomic locus labeling. These data demonstrate that the photoswitchable RBP LicV can serve as a programmable scaffold for the spatiotemporal control of synthetic RNA effectors.
Keyphrases
  • crispr cas
  • nucleic acid
  • genome editing
  • transcription factor
  • electronic health record
  • big data
  • genome wide
  • deep learning
  • radiation induced
  • genome wide association study