Antimicrobial peptides sourced from post-butter processing waste yak milk protein hydrolysates.
Jinjin PeiHai JiangXinsheng LiWengang JinYanduo TaoPublished in: AMB Express (2017)
Yak butter is one of the most important foods for the Tibetan people. Of note, its production yields waste yak milk as a by-product. In this work, waste yak milk protein hydrolysates made via Pepsin hydrolysis were shown to have antimicrobial activity. Furthermore, an innovative method of magnetic liposome adsorption combined with reversed-phase high performance liquid chromatography (RP-HPLC) was developed to screen for and purify the antimicrobial peptides. Two antimicrobial peptides were obtained and their amino acid sequences were determined by N-sequencing, namely Arg-Val-Met-Phe-Lys-Trp-Ala and Lys-Val-Ile-Ser-Met-Ile. The antimicrobial activity spectra of Arg-Val-Met-Phe-Lys-Trp-Ala included Bacillus subtilis, Staphylcoccus aureus, Listeria innocua, Escherichia coli, Enterobacter cloacae and Salmonella paratyphi, while the Lys-Val-Ile-Ser-Met-Ile peptide shows not only bacterial growth inhibition but also of fungi. Haemolytic testing suggested that these two antimicrobial peptides could be considered to have no haemolytic effect at their minimum inhibitory concentrations (MICs).
Keyphrases
- high performance liquid chromatography
- amino acid
- escherichia coli
- tyrosine kinase
- simultaneous determination
- bacillus subtilis
- tandem mass spectrometry
- heavy metals
- solid phase extraction
- mass spectrometry
- sewage sludge
- municipal solid waste
- ms ms
- life cycle
- protein protein
- high throughput
- molecularly imprinted
- liquid chromatography
- high resolution
- small molecule
- density functional theory
- pseudomonas aeruginosa
- klebsiella pneumoniae