Evaluation of the Pathogenic-Mixed Biofilm Formation of Pseudomonas aeruginosa / Staphylococcus aureus and Treatment with Limonene on Three Different Materials by a Dynamic Model.

These data showed that the limonene treatment expressed different levels of biofilm-forming genes, especially when both types of strains alone and together grew on different surfaces. Our findings showed that limonene treatment is also very efficient when biofilm has been grown under shear stress causing significant and irreversible damage to the biofilm structure. The effectiveness of the sanitation procedures can be optimized by applying antimicrobial combinations with natural compounds (e.g., limonene).