LIPRNAseq: a method to discover lipid interacting RNAs by sequencing.
Andrea Bayona-HernandezSusana GuerraIrma Angélica Jiménez-RamirezMartin SztachoPavel HozakLuis Carlos Rodriguez-ZapataAlejandro Pereira-SantanaEnrique CastañoPublished in: Molecular biology reports (2023)
The results show several RNAs differentially enriched in the pull-down of phosphatidyl Inositol 4,5 bisphosphate coated beads. This method is helpful to screen lipid-binding RNA, which may have relevant biological functions. The method can be used with different lipids and comparison of pull-downs and can narrow the selection of RNAs that interact with a particular lipid for further studies.