The interplay of post-translational protein modifications in Arabidopsis leaves during photosynthesis induction.
Jonas GieseJürgen EirichDirk WaltherYoujun ZhangInes LassowskatAlisdair Robert FernieMarlene ElsässerVerónica G MaurinoMarkus SchwarzländerIris FinkemeierPublished in: The Plant journal : for cell and molecular biology (2023)
Diurnal dark to light transition causes profound physiological changes in plant metabolism. These changes require distinct modes of regulation as a unique feature of photosynthetic lifestyle. The activities of several key metabolic enzymes are regulated by light-dependent post-translational modifications (PTM) and have been studied at depth at the level of individual proteins. In contrast, a global picture of the light-dependent PTMome dynamics is lacking, leaving the response of a large proportion of cellular function undefined. Here, we investigated the light-dependent metabolome and proteome changes in Arabidopsis rosettes in a time resolved manner to dissect their kinetic interplay, focusing on phosphorylation, lysine acetylation, and cysteine-based redox switches. Of over 24 000 PTM sites that were detected, more than 1700 were changed during the transition from dark to light. While the first changes, as measured 5 min after onset of illumination, occurred mainly in the chloroplasts, PTM changes at proteins in other compartments coincided with the full activation of the Calvin-Benson cycle and the synthesis of sugars at later timepoints. Our data reveal connections between metabolism and PTM-based regulation throughout the cell. The comprehensive multiome profiling analysis provides unique insight into the extent by which photosynthesis reprograms global cell function and adds a powerful resource for the dissection of diverse cellular processes in the context of photosynthetic function.
Keyphrases
- single cell
- transcription factor
- magnetic resonance
- cardiovascular disease
- metabolic syndrome
- computed tomography
- physical activity
- stem cells
- cell therapy
- electronic health record
- amino acid
- big data
- cell wall
- genome wide
- intellectual disability
- gene expression
- high resolution
- autism spectrum disorder
- protein protein
- plant growth
- single molecule