AtRKD5 inhibits the parthenogenic potential mediated by AtBBM.
Qiyan LiuDongfen HanDenghu ChengJinfan ChenShujuan TianJiafa WangMan LiuLi YuanPublished in: Journal of integrative plant biology (2024)
Parthenogenesis, the development of unfertilized egg cells into embryos, is a key component of apomixis. AtBBM (BABY BOOM), a crucial regulator of embryogenesis in Arabidopsis, possesses the capacity to shift nutritional growth toward reproductive growth. However, the mechanisms underlying AtBBM-induced parthenogenesis remain largely unexplored in dicot plants. Our findings revealed that in order to uphold the order of sexual reproduction, the embryo-specific promoter activity of AtBBM as well as repressors that inhibit its expression in egg cells combine to limiting its ability to induce parthenogenesis. Notably, AtRKD5, a RWP-RK domain-containing (RKD) transcription factor, binds to the 3' end of AtBBM and is identified as one of the inhibitory factors for AtBBM expression in the egg cell. In the atrkd5 mutant, we successfully achieved enhanced ectopic expression of AtBBM in egg cells, resulting in the generation of haploid offspring via parthenogenesis at a rate of 0.28%. Furthermore, by introducing chimeric Arabidopsis and rice BBM genes into the egg cell, we achieved a significant 4.6-fold enhancement in haploid induction through the atdmp8/9 mutant. These findings lay a strong foundation for further exploration of the BBM-mediated parthenogenesis mechanism and the improvement of haploid breeding efficiency mediated by the dmp8/9 mutant.
Keyphrases
- transcription factor
- induced apoptosis
- poor prognosis
- cell cycle arrest
- cell therapy
- single cell
- genome wide identification
- signaling pathway
- stem cells
- metabolic syndrome
- binding protein
- long non coding rna
- cell proliferation
- mental health
- type diabetes
- embryonic stem cells
- dna binding
- high fat diet
- high glucose
- risk assessment
- diabetic rats
- climate change
- pi k akt