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Integrated analysis of biparental and natural populations reveals CRIB domain-containing protein underlying seed coat crack trait in watermelon.

Qi MiHongqian PangFeishi LuanPeng GaoShi Liu
Published in: TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik (2023)
plants narrowed the scc locus to a 277.11 kb region containing 37 candidate genes. Due to the lack of molecular markers in the fine-mapping interval, we extracted the genome sequence variations in this 277.11 kb region with in silico BSA among seventeen re-sequenced lines (6 scc and 11 non-scc) and finally delimited the scc locus to an 8.34 kb region with only one candidate gene Cla97C03G056110 (CRIB domain-containing protein). Three single nucleotide polymorphism loci in the promoter region of Cla97C03G056110 altered cis-acting elements that were highly correlated with the nature watermelon panel. The expression of Cla97C03G056110 in seed coat tissue was higher in non-scc than in scc lines and was specifically expressed in seed coat compared with fruit flesh.
Keyphrases
  • genome wide
  • poor prognosis
  • amino acid
  • binding protein
  • genome wide association study
  • high resolution
  • transcription factor
  • copy number
  • long non coding rna
  • genome wide identification
  • single molecule