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Nourseothricin N-acetyl transferase (NAT), a new selectable marker for nuclear gene expression in Chlamydomonas.

Xinjia YangJialin PengJunmin Pan
Published in: Plant methods (2019)
This work represents the first demonstration of stable expression of NAT in the nuclear genome of C. reinhardtii and provides evidence that NAT can be used as an effective selectable marker for transgenic strains. It provides alterative choice for selectable markers in C. reinhardtii. NAT is compatible with paromomycin and hygromycin B resistance genes, which allows for multiple selections.
Keyphrases
  • gene expression
  • genome wide
  • poor prognosis
  • dna methylation
  • escherichia coli
  • binding protein
  • long non coding rna