Fingerprint of Circulating Immunocytes as Biomarkers for the Prognosis of Brain Inflammation and Neuronal Injury after Cardiac Arrest.
Huanyu DouNicole R BrandonKerryann E KoperYan XuPublished in: ACS chemical neuroscience (2023)
Cardiac arrest is one of the most dangerous health problems in the world. Outcome prognosis is largely based on cerebral performance categories determined by neurological evaluations. Few systemic tests are currently available to predict survival to hospital discharge. Here, we present the results from the preclinical studies of cardiac arrest and resuscitation (CAR) in mice to identify signatures of circulating immune cells as blood-derived biomarkers to predict outcomes after CAR. Two flow cytometry panels for circulating blood lymphocytes and myeloid-derived cells, respectively, were designed to correlate with neuroinflammation and neuronal and dendritic losses in the selectively vulnerable regions of bilateral hippocampi. We found that CD4 + CD25 + regulatory T cells, CD11b + CD11c - and CD11b + Ly6C + Ly6G + myeloid-derived cells, and cells positive for the costimulatory molecules CD80 and CD86 in the blood were correlated with activation of microglia and astrocytosis, and CD4 + CD25 + T cells are additionally correlated with neuronal and dendritic losses. A fingerprint pattern of blood T cells and monocytes is devised as a diagnostic tool to predict CAR outcomes. Blood tests aimed at identifying these immunocyte patterns in cardiac arrest patients will guide future clinical trials to establish better prognostication tools to avoid unnecessary early withdrawal from life-sustaining treatment.
Keyphrases
- cardiac arrest
- cardiopulmonary resuscitation
- induced apoptosis
- regulatory t cells
- cerebral ischemia
- dendritic cells
- clinical trial
- cell cycle arrest
- nk cells
- mental health
- flow cytometry
- oxidative stress
- healthcare
- signaling pathway
- end stage renal disease
- stem cells
- type diabetes
- randomized controlled trial
- chronic kidney disease
- metabolic syndrome
- newly diagnosed
- subarachnoid hemorrhage
- peripheral blood
- endoplasmic reticulum stress
- genome wide
- spinal cord injury
- adipose tissue
- neuropathic pain
- prognostic factors
- insulin resistance
- cell death
- ejection fraction
- lipopolysaccharide induced
- skeletal muscle
- blood brain barrier
- brain injury
- functional connectivity
- health information
- peritoneal dialysis
- white matter
- current status
- weight loss