Sensitive detection of specific cell-free DNA in serum samples from sheep with cystic echinococcosis.

Mahboubeh HadipourHossein Yousofi DaraniHamid TalebzadehMohammad EslamianShima AboutalebianMajid Fasihi HarandiHossein Mirhendi

Published in: PLoS neglected tropical diseases (2023)

Larger volumes of serum for DNA extraction, greater volumes of DNA template for PCR, and employing a semi-nested PCR protocol, increased the sensitivity of PCR to 95%. This approach can also be applied to the diagnosis of echinococcosis in humans.

Keyphrases

sensitive detection
circulating tumor
cell free
single molecule
real time pcr
quantum dots
randomized controlled trial
nucleic acid
molecularly imprinted
high resolution
circulating tumor cells