Generation of double knockout cattle via CRISPR-Cas9 ribonucleoprotein (RNP) electroporation.
Gyeong-Min GimKyeong-Hyeon EomDong-Hyeok KwonDae-Jin JungDae-Hyun KimJun-Koo YiJae-Jung HaJi-Hyun LeeSeong-Beom LeeWoo-Jae SonSoo-Young YumWon-Wu LeeGoo JangPublished in: Journal of animal science and biotechnology (2023)
These data demonstrated that production of genome edited cattle via electroporation of RNP could be effectively applied. Finally, MSTN and PRNP from beef cattle and MSTN and BLG from dairy cattle have been born and they will be valuable resources for future precision breeding.