Tetrandrine Attenuates Podocyte Injury by Inhibiting TRPC6-Mediated RhoA/ROCK1 Pathway.
Lichan MaoYin DingDongrong YuJiazhen YinJin YuPublished in: Analytical cellular pathology (Amsterdam) (2022)
Tetrandrine (Tet), a compound found in a traditional Chinese medicine, presents the protective effect for kidney function. Our study is aimed at clarifying the efficacy and underlying mechanism of Tet on podocyte injury. In this study, podocyte injury was induced in rats with adriamycin (ADR), and MPC5 podocytes were constructed with TRPC6 overexpression. We found that Tet treatment reduced the levels of proteinuria, serum creatinine, and blood urea nitrogen and increased plasma albumin levels in ADR-induced rats. Tet reduced intracellular Ca 2+ influx and apoptosis in MPC5 podocytes overexpressing TRPC6. Tet downregulated the expression of renal TRPC6, RhoA, and ROCK1 and upregulated the expression of synaptopodin; meanwhile, it reduced calcineurin activity in vivo and in vitro . In conclusion, Tet protects against podocyte by affecting TRPC6 and its downstream RhoA/ROCK1 signaling pathway.
Keyphrases
- high glucose
- endothelial cells
- diabetic nephropathy
- signaling pathway
- vascular smooth muscle cells
- poor prognosis
- diabetic rats
- cell proliferation
- adverse drug
- emergency department
- epithelial mesenchymal transition
- cell death
- wastewater treatment
- binding protein
- transcription factor
- drug induced
- angiotensin ii
- uric acid
- combination therapy
- reactive oxygen species