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Assessment of the ptxD gene as a growth and selective marker in Trichoderma atroviride using Pccg6, a novel constitutive promoter.

Nohemí Carreras-VillaseñorJosé Guillermo Rico-RuizRicardo A Chávez MontesLenin Yong-VillalobosJosé Fabricio López-HernándezPedro Martínez-HernándezLuis Herrera-EstrellaAlfredo Herrera-EstrellaDamar Lizbeth López-Arredondo
Published in: Microbial cell factories (2020)
A new constitutive promoter, ccg6, for expression of homologous and heterologous proteins has been identified and tested in T. atroviride to express PTXD, which resulted in an effective and visible phenotype to evaluate transcriptional activity of sequence promoters. Use of PTXD as a growth marker holds great potential for assessing activity of other promoters and for biotechnological applications as a contamination control system.
Keyphrases
  • transcription factor
  • gene expression
  • dna methylation
  • poor prognosis
  • risk assessment
  • human health
  • genome wide
  • dna damage
  • drinking water
  • copy number
  • health risk
  • binding protein
  • genome wide identification
  • amino acid