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Getting value from the waste: recombinant production of a sweet protein by Lactococcus lactis grown on cheese whey.

Mohamed BoumaizaAndrea ColarussoErmenegilda ParrilliElena Garcia-FruitósAngela CasilloAnna ArísMaria Michela CorsaroDelia PiconeSerena LeoneMaria Luisa Tutino
Published in: Microbial cell factories (2018)
Other than presenting a new system for the recombinant production of MNEI, more compliant with its potential applications in food industry, our results introduce a strategy to valorize dairy effluents through the synthesis of high added value recombinant proteins. Interestingly, the possibility of using this whey-derived medium relied greatly on the choice of the appropriate codon usage for the target gene. In fact, when a gene optimized for L. lactis was used, the production of MNEI proceeded with good yields. On the other hand, when an E. coli optimized gene was employed, protein synthesis was greatly reduced, to the point of being completely abated in the cheese whey-based medium. The production of MMP-9 was comparable to what observed in the reference conditions.
Keyphrases
  • genome wide
  • copy number
  • genome wide identification
  • escherichia coli
  • cell free
  • heavy metals
  • dna methylation
  • protein protein
  • binding protein
  • lactic acid