Generation of the salicylic acid deficient Arabidopsis via a synthetic salicylic acid hydroxylase expression cassette.
Zilin CaiHao GuoShijing ShenQilu YuJinbin WangEngao ZhuPinghua ZhangLili SongYanjun ZhangKewei ZhangPublished in: Plant methods (2022)
Compared with NahG transgenic lines producing by-product catechol, S5Hpro::EGFP-S3H transgenic lines reduce SA levels by converting SA to a native product 2,3-DHBA for catabolism. Together, we provide new SA-deficient germplasms for the investigations of SA signaling in plant development, leaf senescence, and disease resistance.