Efficient immunoaffinity chromatography of lymphocytes directly from whole blood.
Fabian MohrSabine PrzibillaFranziska LeonhardtChristian StembergerStefan DreherThomas R MüllerSimon P FräßleGeorg P SchmidtMarie-Luise KieneHerbert StadlerDirk H BuschPublished in: Scientific reports (2018)
We show that defined lymphocytes can be rapidly purified by immunoaffinity chromatography starting directly from whole blood. The method relies on low-affinity Fab-fragments attached to a column-matrix combined with the reversible Strep-tag technology. Compared to established cell enrichment protocols, the Strep-tag affinity chromatography of cells is independent of erythrocyte lysis or centrifugation steps, allowing for simple cell-enrichment with good yields, high purities, and excellent functionality of purified cells.
Keyphrases
- induced apoptosis
- mass spectrometry
- liquid chromatography
- high speed
- cell cycle arrest
- tandem mass spectrometry
- single cell
- high performance liquid chromatography
- cell therapy
- peripheral blood
- endoplasmic reticulum stress
- stem cells
- oxidative stress
- signaling pathway
- high resolution
- bone marrow
- mesenchymal stem cells
- pi k akt
- ms ms