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Efficient immunoaffinity chromatography of lymphocytes directly from whole blood.

Fabian MohrSabine PrzibillaFranziska LeonhardtChristian StembergerStefan DreherThomas R MüllerSimon P FräßleGeorg P SchmidtMarie-Luise KieneHerbert StadlerDirk H Busch
Published in: Scientific reports (2018)
We show that defined lymphocytes can be rapidly purified by immunoaffinity chromatography starting directly from whole blood. The method relies on low-affinity Fab-fragments attached to a column-matrix combined with the reversible Strep-tag technology. Compared to established cell enrichment protocols, the Strep-tag affinity chromatography of cells is independent of erythrocyte lysis or centrifugation steps, allowing for simple cell-enrichment with good yields, high purities, and excellent functionality of purified cells.
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